{"id":111363,"date":"2019-05-22T09:47:05","date_gmt":"2019-05-22T01:47:05","guid":{"rendered":"http:\/\/www.fagao.me\/?p=111363"},"modified":"2019-05-22T09:47:05","modified_gmt":"2019-05-22T01:47:05","slug":"%e5%a6%82%e4%bd%95%e5%9c%a8%e6%95%b0%e6%8d%ae%e5%ba%93%e4%b8%ad%e6%90%9c%e7%b4%a2%e4%bb%a3%e8%b0%a2%e7%89%a9","status":"publish","type":"post","link":"https:\/\/www.fagao.me\/p\/111363.htm","title":{"rendered":"\u5982\u4f55\u5728\u6570\u636e\u5e93\u4e2d\u641c\u7d22\u4ee3\u8c22\u7269"},"content":{"rendered":"
<\/div>\n

\u6700\u8fd1\u5341\u51e0\u5e74\u4ee3\u8c22\u7ec4\u6280\u672f\u53d1\u5c55\u8fc5\u901f\uff0c\u4f46\u6570\u636e\u5e93\u4ecd\u7136\u662f\u6700\u5927\u7684\u5236\u7ea6\u56e0\u7d20\u4e4b\u4e00\uff0c\u6570\u636e\u5e93\u7684\u4e0d\u5168\u9762\u5bfc\u81f4\u4e86\u80fd\u591f\u68c0\u6d4b\u9274\u5b9a\u51fa\u6765\u7684\u7269\u8d28\u6570\u76ee\u4e0d\u591f\u4e30\u5bcc\uff0c\u7279\u522b\u662fLC-MS\uff0c\u6570\u636e\u5e93\u66f4\u52a0\u4e0d\u5065\u5168\u3002\u800c\u6211\u4eec\u901a\u5e38\u5e0c\u671b\u975e\u9776\u5411\u4ee3\u8c22\u7ec4\u68c0\u6d4b\u80fd\u591f\u9274\u5b9a\u51fa\u6211\u4eec\u611f\u5174\u8da3\u7684\u51e0\u79cd\u5341\u51e0\u79cd\u7269\u8d28\uff0c\u90a3\u4e48\u6211\u4eec\u53ef\u4ee5\u641c\u7d22\u4e00\u4e0b\u5e38\u7528\u7684\u4ee3\u8c22\u7269\u6570\u636e\u5e93\uff0c\u505a\u5230\u5fc3\u91cc\u6709\u5e95\u554a\u3002<\/span><\/p>\n<\/p>\n

\u4eca\u5929\u5c31\u7ed9\u5927\u5bb6\u4ecb\u7ecd\u4e00\u4e0b\u5982\u4f55\u5728METLIN\u6570\u636e\u5e93\u4e2d\u641c\u7d22\u611f\u5174\u8da3\u7684\u7269\u8d28<\/strong>\uff0cMETLIN\u6570\u636e\u5e93<\/strong><\/span>\u4e0eHMDB\u6570\u636e\u5e93<\/strong><\/span>\u90fd\u662f\u6700\u5e38\u7528\u7684\u4ee3\u8c22\u7269\u6570\u636e\u5e93\uff0c\u4e00\u822c\u5728\u4ee3\u8c22\u7ec4\u516c\u53f8\u7684\u5b9a\u6027\u4e2d\u90fd\u4f1a\u7528\u5230\u7684\u3002<\/span><\/p>\n

METLIN\u6570\u636e\u5e93\uff08https:\/\/metlin.scripps.edu<\/span>\uff09\u662f\u7531\u7f8e\u56fd\u65af\u514b\u91cc\u666e\u65af\u7814\u7a76\u9662\u5f00\u53d1\u7684\uff0c\u662f\u4e00\u4e2a\u975e\u5e38\u5168\u9762\u7684\u8d28\u8c31\u548c\u4e8c\u7ea7\u8d28\u8c31\u6570\u636e\u5e93\uff0c\u8fd9\u4e9b\u4e8c\u7ea7\u8d28\u8c31\u6570\u636e\u662f\u5728\u591a\u4e2aQTOF\u8d28\u8c31\u68c0\u6d4b\u5e73\u53f0\u5f97\u5230\u7684\uff0c\u5305\u62ecSCIEX\u3001Agilent\u3001Bruker\u3001Waters\uff0c\u5305\u62ec\u4e86\u5728\u4e0d\u540c\u7684\u78b0\u649e\u80fd\u3001\u6b63\u8d1f\u79bb\u5b50\u6a21\u5f0f\u4e0b\u5f97\u5230\u7684\u4e8c\u7ea7\u8c31\u56fe\u3002<\/span><\/p>\n

METLIN\u6570\u636e\u5e93\u5305\u542b\u4e86961,829\u4e2a\u7269\u8d28\uff0c\u79cd\u7c7b\u6db5\u76d6\u8102\u8d28\u3001\u7c7b\u56fa\u9187\u3001\u690d\u7269\u4ee3\u8c22\u7269\u3001\u5fae\u751f\u7269\u4ee3\u8c22\u7269\u3001\u77ed\u80bd\u3001\u78b3\u6c34\u5316\u5408\u7269\u3001\u5916\u6e90\u836f\u7269\/\u5316\u5408\u7269\u3001\u6709\u6bd2\u7269\u8d28\u7b49\u7b49\u3002<\/span><\/p>\n

METLIN\u6570\u636e\u5e93\u53ef\u8fdb\u884c\u591a\u79cd\u641c\u7d22\uff0c\u5305\u62ec\u5355\u4e2a\u7269\u8d28\u641c\u7d22\u3001\u6279\u91cf\u641c\u7d22\u3001\u6839\u636e\u6bcd\u79bb\u5b50\u3001\u4e2d\u6027\u4e22\u5931\u3001\u5206\u5b50\u91cf\u3001\u4e8c\u7ea7\u788e\u7247\u79bb\u5b50\u7b49\u4fe1\u606f\u8fdb\u884c\u641c\u7d22\uff0c\u5e76\u63d0\u4f9b\u4e30\u5bcc\u7684\u7269\u8d28\u4fe1\u606f\uff0c\u5305\u62ec\u540d\u79f0\u3001\u7ed3\u6784\u3001\u5206\u5b50\u5f0f\u3001\u5206\u5b50\u91cf\u3001CAS\u53f7\u3001KEGG ID\u548c\u94fe\u63a5\u3001HMDB ID\u548c\u94fe\u63a5\u3001PubChem ID\u548c\u94fe\u63a5\u7b49\u7b49\u3002<\/span><\/p>\n

\u4e0b\u9762\u6211\u4eec\u5c31\u6765\u770b\u770b\u5982\u4f55\u5728METLIN\u6570\u636e\u5e93\u91cc\u641c\u7d22\u6211\u4eec\u611f\u5174\u8da3\u7684\u7269\u8d28\u3002<\/span><\/p>\n

01<\/p>\n

\u767b\u5f55\u7f51\u5740\uff08http:\/\/metlin.scripps.edu\/index.php<\/span>\uff09\uff0c\u5e76\u6ce8\u518c\uff08\u9700\u8981\u6ce8\u518c\u624d\u80fd\u8fdb\u53bb<\/span>\uff09\uff1a<\/span><\/p>\n<\/p>\n

02<\/p>\n

\u70b9\u51fb\u201cadvanced search\u201d\u540e\u8fdb\u5165\u68c0\u7d22\u754c\u9762\uff0c\u53f3\u8fb9\u5217\u51fa\u4e86\u4e00\u4e9b\u68c0\u7d22\u9009\u9879\uff0c\u4e0d\u9700\u8981\u5168\u90e8\u90fd\u586b\uff0c\u586b\u4e00\u4e2a\u6216\u51e0\u4e2a\u90fd\u53ef\u4ee5\u7684\u3002\u6240\u4ee5\u77e5\u9053\u4ec0\u4e48\u4fe1\u606f\u5c31\u586b\u4ec0\u4e48\u4fe1\u606f\u5427\u3002<\/span><\/p>\n<\/p>\n

03<\/p>\n

\u4f8b\u5982\uff0c\u6211\u60f3\u77e5\u9053Methyl jasmonate\uff08\u7532\u57fa\u8309\u8389\u9178<\/span>\uff09\u8fd9\u4e2a\u7269\u8d28\u5728METLIN\u6570\u636e\u5e93\u91cc\u6709\u6ca1\u6709\uff0c\u5c31\u53ef\u4ee5\u5728\u201cName\u201d\u8fd9\u91cc\u8f93\u5165\u82f1\u6587\u540d\u5b57\u3002\u5982\u679c\u60f3\u8981\u7cbe\u786e\u5339\u914d\uff0c\u5219\u53ef\u4ee5\u52fe\u9009\u4e0b\u9762\u7684\u201cName Exact Match\u201d\u9009\u9879\uff1b\u5982\u679c\u5bf9\u4e8e\u60f3\u8981\u67e5\u7684\u7269\u8d28\u7684\u82f1\u6587\u540d\u4e0d\u662f\u592a\u786e\u5b9a\uff0c\u5c31\u4e0d\u7528\u52fe\u9009\u8fd9\u4e2a\u9009\u9879\uff0c\u90a3\u4e48\u4e0e\u8fd9\u4e2a\u540d\u5b57\u76f8\u5173\u7684\u7269\u8d28\u90fd\u4f1a\u88ab\u641c\u51fa\u6765\u3002\u6700\u540e\uff0c\u70b9\u51fb\u4e0b\u65b9\u7684\u201csearch\u201d\uff0c\u8fdb\u884c\u641c\u7d22\u3002<\/span><\/p>\n<\/p>\n

04<\/p>\n

\u641c\u7d22\u7ed3\u679c\uff1a\u641c\u51fa\u6765\u4e24\u4e2a\u7269\u8d28\uff0c\u5206\u522b\u7ed9\u51fa\u4e86METLIN ID\u3001\u5206\u5b50\u91cf\u3001\u82f1\u6587\u540d\u3001\u5206\u5b50\u5f0f\u3001CAS\u53f7\u3001KEGG ID\u3001MS\/MS\u548c\u5206\u5b50\u7ed3\u6784\u4fe1\u606f\u3002<\/span><\/p>\n<\/p>\n

1\uff09METLIN ID\uff1a<\/span><\/strong><\/span>\u70b9\u51fb\u8fdb\u53bb\uff0c\u5c31\u4f1a\u663e\u793a\u4e0b\u9762\u8fd9\u4e9b\u4fe1\u606f\uff1a<\/span><\/p>\n<\/p>\n

2\uff09KEGG\uff1a<\/span><\/strong><\/span>\u70b9\u51fbKEGG ID\uff08C\u53f7<\/span>\uff09\uff0c\u5c31\u53ef\u4ee5\u8df3\u8f6c\u5230KEGG\u5b98\u7f51\uff0c\u67e5\u770b\u76f8\u5e94\u7684\u4fe1\u606f\u3002<\/span><\/p>\n<\/p>\n

3\uff09MS\/MS\uff1a<\/span><\/strong><\/span>\u662f\u8be5\u7269\u8d28\u7684\u4e8c\u7ea7\u788e\u7247\u79bb\u5b50\u56fe\u8c31\u548c\u4fe1\u606f\u3002METLIN\u7ed9\u51fa\u4e86\u5728\u4e0d\u540c\u7684\u78b0\u649e\u80fd\uff08Collision Energy\uff0cCV<\/span>\uff09\u4e0b\u7684\u4e8c\u7ea7\u788e\u7247\u79bb\u5b50\u56fe\u8c31\u3002\u5982\u70b9\u51fb\u7b2c\u4e8c\u884cMethyl jasmonate\u7684MS\/MS View\u8fdb\u53bb\u540e\uff0c\u80fd\u591f\u770b\u5230\u7ed9\u51fa\u4e86\u56db\u79cd\u78b0\u649e\u80fd\u4e0b\u7684\u4e8c\u7ea7\u8c31\u56fe\uff0c\u8c31\u56fe\u4e0a\u9762\u5217\u51fa\u4e86\u8d28\u8c31\u6240\u4f7f\u7528\u7684\u4eea\u5668\u3001\u79bb\u5b50\u6e90\u548c\u6a21\u5f0f\uff0c\u4e0b\u65b9\u5217\u51fa\u4e86\u56db\u79cd\u78b0\u649e\u80fd\uff0c\u70b9\u51fb\u5373\u53ef\u663e\u793a\u76f8\u5e94\u7684\u4e8c\u7ea7\u8c31\u56fe\u3002\u5728\u8fd9\u4e2a\u4e8c\u7ea7\u8c31\u56fe\u4e0a\uff0c\u9f20\u6807\u79fb\u5230\u4fe1\u53f7\u5cf0\u4e0a\u9762\uff0c\u5c31\u4f1a\u663e\u793a\u6a21\u5f0f\u3001\u78b0\u649e\u80fd\u3001\u8d28\u8377\u6bd4\u3001\u79bb\u5b50\u5f3a\u5ea6\u7b49\u7684\u4fe1\u606f\uff0c\u5982\u4e0b\u9762\u6240\u793a\uff1a<\/span><\/p>\n<\/p>\n

\u6211\u4eec\u70b9\u51fb\u201c0V\u201d\u67e5\u770b\u76f8\u5e94\u7684\u8c31\u56fe\uff0c\u53d1\u73b0\u53ea\u6709\u4e00\u4e2a\u5f3a\u5ea6\u4e3a100%\u7684\u5cf0\uff0c\u8fd9\u662f\u56e0\u4e3a0V\u8868\u793a\u6ca1\u6709\u8fdb\u884c\u4e8c\u7ea7\u78b0\u649e\uff0c\u8fd9\u4e2a\u5cf0\u5c31\u662f\u76f8\u5e94\u7684\u6bcd\u79bb\u5b50\u7684\u5cf0\uff0c\u6240\u4ee5\u8d28\u8377\u6bd4\u4e3a225.148\uff08M+H<\/span>\uff09\uff1a<\/span><\/p>\n<\/p>\n

\u70b9\u51fb10V\u300120V\u3001\u548c40V\u770b\u770b\u76f8\u5e94\u7684\u8c31\u56fe\uff0c\u53d1\u73b0\u78b0\u649e\u80fd\u8d8a\u9ad8\uff0c\u5f97\u5230\u7684\u788e\u7247\u79bb\u5b50\u8d8a\u591a\u8d8a\u788e\uff0c\u5927\u5bb6\u53ef\u4ee5\u5bf9\u6bd4\u4e0b\u3002<\/span><\/p>\n

\u7136\u540e\uff0c\u518d\u8bd5\u4e00\u4e2a\u7269\u8d28\uff0c\u770b\u770bMETLIN\u6570\u636e\u4e2d\u6709\u6ca1\u6709\u4e39\u53c2\u915a\u9178B\u8fd9\u79cd\u7269\u8d28\u3002\u8f93\u5165\u82f1\u6587\u540d\u79f0\uff0c\u7ed3\u679c\u663e\u793a\u6570\u636e\u5e93\u4e2d\u5e76\u6ca1\u6709\u8fd9\u4e2a\u7269\u8d28\uff1a<\/span><\/p>\n<\/p>\n

Simple search\u6a21\u5757\uff1a<\/span><\/strong><\/span><\/p>\n

\u8fd9\u4e2a\u6a21\u5757\u662f\u6839\u636e\u5206\u5b50\u91cf\u3001PPM\u3001\u68c0\u6d4b\u6a21\u5f0f\u3001\u52a0\u5408\u7269\u7b49\u4fe1\u606f\u8fdb\u884c\u7b5b\u9009\u7684\uff0c\u5982\u679c\u6211\u4eec\u6709\u8d28\u8c31\u6570\u636e\uff0c\u9700\u8981\u8fdb\u884c\u7269\u8d28\u5b9a\u6027\uff0c\u90a3\u4e48\u53ef\u4ee5\u4f7f\u7528\u8fd9\u4e2a\u6a21\u5757\u3002\u4f46\u662f\u901a\u5e38\u90fd\u4f1a\u5339\u914d\u51fa\u6765\u591a\u4e2a\u7269\u8d28\uff08\u5982\u4e0b\u56fe<\/span>\uff09\uff0c\u90a3\u4e48\u8fdb\u4e00\u6b65\u5c31\u9700\u8981\u81ea\u5df1\u7684\u4e13\u4e1a\u77e5\u8bc6\u53bb\u8fdb\u884c\u7b5b\u9009\u548c\u5224\u65ad\u4e86\u3002<\/span><\/p>\n<\/p>\n

METLIN\u6570\u636e\u5e93\u8fd8\u6709\u5176\u4ed6\u7684\u6a21\u5757\uff0c\u5982\u6279\u91cf\u641c\u7d22\u3001\u788e\u7247\u641c\u7d22\u3001\u4e2d\u6027\u4e22\u5931\u641c\u7d22\u3001\u4e8c\u7ea7\u8c31\u56fe\u641c\u7d22\u7b49\uff0c\u5927\u5bb6\u90fd\u53ef\u4ee5\u53bb\u8bd5\u8bd5\u3002<\/span><\/p>\n

\u597d\u7684\uff0c\u4eca\u5929\u7684METLIN<\/span>\u6570\u636e\u5e93\u4ecb\u7ecd\u5c31\u5230\u8fd9\uff0c\u5e0c\u671b\u5bf9\u5927\u5bb6\u6709\u7528\u54c8\u3002<\/span><\/p>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

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